An enhanced fat extraction from Pycnanthus angolensis (African nutmeg) seeds using cellulase from Aspergillus niger strain BC23

A promising extraction method for obtaining fat from Pycnanthus angolensis seeds through Aspergillus niger strain BC23 enzyme pretreatment and particle size reduction is presented in this work. The employed A. was produced corn bagasse waste material, which served as the only source of carbon. P. were dried, pulverized subsequently sieved into two different sizes 1.0 mm 1.4 mm. This followed by prior to extraction. Results demonstrated that while peak activity (121.036 U/mL) occurred on day 4 fermentation, maximum cellulase could be achieved at 60% saturation ammonium sulfate purification. Compared with results water-treated untreated seed samples, yield, free fatty acid iodine values enzyme-treated samples considerably higher (P < 0.05). As peroxide value less enzyme-assisted extracted fat, pointed a good-quality oil, decreased refractive index suggested unsaturation. Despite considerable differences 0.05) indices, fat’s specific gravity matched > when enzyme-treated, compared. On whole, pretreated improve both quality yield implied it useful inventory confectionary well cosmetic industries.